학술논문
Bacterial biogeography of adult airways in atopic asthma
Document Type
article
Author
Juliana Durack; Yvonne J. Huang; Snehal Nariya; Laura S. Christian; K. Mark Ansel; Avraham Beigelman; Mario Castro; Anne-Marie Dyer; Elliot Israel; Monica Kraft; Richard J. Martin; David T. Mauger; Sharon R. Rosenberg; Tonya S. King; Steven R. White; Loren C. Denlinger; Fernando Holguin; Stephen C. Lazarus; Njira Lugogo; Stephen P. Peters; Lewis J. Smith; Michael E. Wechsler; Susan V. Lynch; Homer A. Boushey; for the National Heart, Lung and Blood Institute’s “AsthmaNet”
Source
Microbiome, Vol 6, Iss 1, Pp 1-16 (2018)
Subject
Language
English
ISSN
2049-2618
Abstract
Abstract Background Perturbations to the composition and function of bronchial bacterial communities appear to contribute to the pathophysiology of asthma. Unraveling the nature and mechanisms of these complex associations will require large longitudinal studies, for which bronchoscopy is poorly suited. Studies of samples obtained by sputum induction and nasopharyngeal brushing or lavage have also reported asthma-associated microbiota characteristics. It remains unknown, however, whether the microbiota detected in these less-invasive sample types reflect the composition of bronchial microbiota in asthma. Results Bacterial microbiota in paired protected bronchial brushings (BB; n = 45), induced sputum (IS; n = 45), oral wash (OW; n = 45), and nasal brushings (NB; n = 27) from adults with mild atopic asthma (AA), atopy without asthma (ANA), and healthy controls (HC) were profiled using 16S rRNA gene sequencing. Though microbiota composition varied with sample type (p