학술논문
Occurrence and diversity of avian haemosporidia in Afrotropical landbirds
Document Type
article
Source
International Journal for Parasitology: Parasites and Wildlife, Vol 8, Iss , Pp 36-44 (2019)
Subject
Language
English
ISSN
2213-2244
Abstract
Avian haemosporidian infections are widespread and can result in the decline of wild bird populations or in some cases contribute to extinction of species. We determined the prevalence and genetic diversity of avian haemosporidia in 93 samples from 22 landbird species from South Africa (N = 76) and West Africa (N = 17), of which six are intra-African migrants and one is a Palearctic migrant. The samples were analysed for the presence of avian haemosporidian DNA using real-time quantitative PCR (qPCR) and nested PCR assays targeting specific mitochondrial genes of these parasites. The cytochrome b (cytb) gene was sequenced for all samples that tested positive and phylogenetic analysis was conducted in order to determine the relationship of the new sequences with previously published sequences from the MalAvi database. The overall prevalence of avian haemosporidiosis was 68.82% (95% CI: 56.4%–78.87%) and 82.80% (95% CI: 65.68%–86.11%) as determined by qPCR and nested PCR respectively. Eighteen (19.36%; 95% CI; 10.78%–29.97%) samples had mixed infections. Infection prevalence of all haemosporidian spp. were significantly higher (p