학술논문
DNA methylation-based profiling of horse archaeological remains for age-at-death and castration
Document Type
article
Author
Xuexue Liu; Andaine Seguin-Orlando; Lorelei Chauvey; Gaëtan Tressières; Stéphanie Schiavinato; Laure Tonasso-Calvière; Jean-Marc Aury; Aude Perdereau; Stefanie Wagner; Pierre Clavel; Oscar Estrada; Jianfei Pan; Yuehui Ma; Jacob Enk; Alison Devault; Jennifer Klunk; Sébastien Lepetz; Benoit Clavel; Lin Jiang; Patrick Wincker; Yvette Running Horse Collin; Clio Der Sarkissian; Ludovic Orlando
Source
iScience, Vol 26, Iss 3, Pp 106144- (2023)
Subject
Language
English
ISSN
2589-0042
Abstract
Summary: Age profiling of archaeological bone assemblages can inform on past animal management practices, but is limited by the fragmentary nature of the fossil record and the lack of universal skeletal markers for age. DNA methylation clocks offer new, albeit challenging, alternatives for estimating the age-at-death of ancient individuals. Here, we take advantage of the availability of a DNA methylation clock based on 31,836 CpG sites and dental age markers in horses to assess age predictions in 84 ancient remains. We evaluate our approach using whole-genome sequencing data and develop a capture assay providing reliable estimates for only a fraction of the cost. We also leverage DNA methylation patterns to assess castration practice in the past. Our work opens for a deeper characterization of past husbandry and ritual practices and holds the potential to reveal age mortality profiles in ancient societies, once extended to human remains.