학술논문
Temporary increase in circulating replication-competent latent HIV-infected resting CD4+ T cells after switch to an integrase inhibitor based antiretroviral regimenResearch in context
Document Type
article
Author
Roux-Cil Ferreira; Steven J. Reynolds; Adam A. Capoferri; Owen R. Baker; Erin E. Brown; Ethan Klock; Jernelle Miller; Jun Lai; Sharada Saraf; Charles Kirby; Briana Lynch; Jada Hackman; Sarah N. Gowanlock; Stephen Tomusange; Samiri Jamiru; Aggrey Anok; Taddeo Kityamuweesi; Paul Buule; Daniel Bruno; Craig Martens; Rebecca Rose; Susanna L. Lamers; Ronald M. Galiwango; Art F.Y. Poon; Thomas C. Quinn; Jessica L. Prodger; Andrew D. Redd
Source
EBioMedicine, Vol 102, Iss , Pp 105040- (2024)
Subject
Language
English
ISSN
2352-3964
Abstract
Summary: Background: The principal barrier to an HIV cure is the presence of the latent viral reservoir (LVR), which has been understudied in African populations. From 2018 to 2019, Uganda instituted a nationwide rollout of ART consisting of Dolutegravir (DTG) with two NRTI, which replaced the previous regimen of one NNRTI and the same two NRTI. Methods: Changes in the inducible replication-competent LVR (RC-LVR) of ART-suppressed Ugandans with HIV (n = 88) from 2015 to 2020 were examined using the quantitative viral outgrowth assay. Outgrowth viruses were examined for viral evolution. Changes in the RC-LVR were analyzed using three versions of a Bayesian model that estimated the decay rate over time as a single, linear rate (model A), or allowing for a change at time of DTG initiation (model B&C). Findings: Model A estimated the slope of RC-LVR change as a non-significant positive increase, which was due to a temporary spike in the RC-LVR that occurred 0–12 months post-DTG initiation (p