학술논문

Analysis of terpenoid biotransformation in beer by commercial Saccharomyces cerevisiae yeast using headspace SPME-GC/MS
Document Type
article
Source
Food Chemistry Advances, Vol 4, Iss , Pp 100692- (2024)
Subject
Terpenoids
Yeast biotransformation
Beer fermentation
Hops
Headspace solid-phase Microextraction gas chromatography mass spectrometry (HS SPME-GC/MS)
Food processing and manufacture
TP368-456
Language
English
ISSN
2772-753X
Abstract
Terpenoids are a class of compounds found in hops which are responsible for the distinctive hop aromas in beer. Changes in terpenoid composition during fermentation as a result of yeast biotransformation influences beer aroma, but the pathways involved in these reactions are yet to be fully understood. In this study, eleven terpenoid standards (geraniol, nerol, citronellol, citral, α-terpineol, citronellyl acetate, caryophyllene, linalool, limonene, β-pinene and myrcene) were individually added to a model beer system fermented with commercially available Saccharomyces cerevisiae yeast (SafAle US-05). Volatile organic compounds (VOCs) were measured before and after fermentation with headspace solid-phase microextraction gas chromatography/mass spectrometry (SPME-GC/MS). Upon adding a single terpenoid standard to the model beer system, multiple terpenoid products were detected in the resulting beer at the end of fermentation. For example, when geraniol (10 ppm) was added, geraniol, citronellol, citronellyl acetate, citronellal, nerol, dihydrolinalool and dihydrocitronellol were detected at the end of fermentation. This research illustrates the importance of studying individual terpenoids as it provides valuable insights into the complex chemistry of beer. This information can aid in the optimisation of brewing to enhance the production of the flavours and aromas in beer desired by consumers.