학술논문
Characterizing carbapenemase-producing Escherichia coli isolates from Spain: high genetic heterogeneity and wide geographical spread
Document Type
article
Author
Elias Dahdouh; Laro Gómez-Marcos; Javier E. Cañada-García; Eva Ramírez de Arellano; Aida Sánchez-García; Isabel Sánchez-Romero; Luis López-Urrutia; Pedro de la Iglesia; Alejandro Gonzalez-Praetorius; Jared Sotelo; Daniel Valle-Millares; Isabela Alonso-González; Verónica Bautista; Noelia Lara; Silvia García-Cobos; Emilia Cercenado; Belén Aracil; Jesús Oteo-Iglesias; María Pérez-Vázquez; Spanish Eco-Carba Study Group; Verónica Casquero; Olga Valiente; Almudena Alhambra Mosquera; Alia Eworo Ndongo; Susana Hernando Real; Luis Moisés Ruiz-Velasco; José Leiva; Nieves Balado; Adriana Ortega; Mar Olga Pérez Moreno; Ana Bordes; Cristobal del Rosario Quintana; María Eugenia Portillo; Caridad Sainz de Baranda; Gloria Trujillo; Begoña Palop; Carmen Aldea-Mansilla; Juan Cuadros; Yolanda Gil; Soledad Illescas Fernández-Bermejo; Ana Ramos; Salvador Giner; Antonio Casabella Pernas; M. Pilar Ortega Lafont; María Huertas Vaquero; Isabel Antolín; Ma de los Ángeles Pallarés; Beatriz Iglesias; Frederic Gómez-Bertomeu; Ana Isabel López-Calleja; Pilar Zamarrón
Source
Frontiers in Cellular and Infection Microbiology, Vol 14 (2024)
Subject
Language
English
ISSN
2235-2988
Abstract
IntroductionCarbapenemase-Producing Escherichia coli (CP-Eco) isolates, though less prevalent than other CP-Enterobacterales, have the capacity to rapidly disseminate antibiotic resistance genes (ARGs) and cause serious difficult-to-treat infections. The aim of this study is phenotypically and genotypically characterizing CP-Eco isolates collected from Spain to better understand their resistance mechanisms and population structure.MethodsNinety representative isolates received from 2015 to 2020 from 25 provinces and 59 hospitals Spanish hospitals were included. Antibiotic susceptibility was determined according to EUCAST guidelines and whole-genome sequencing was performed. Antibiotic resistance and virulence-associated genes, phylogeny and population structure, and carbapenemase genes-carrying plasmids were analyzed.Results and discussionThe 90 CP-Eco isolates were highly polyclonal, where the most prevalent was ST131, detected in 14 (15.6%) of the isolates. The carbapenemase genes detected were blaOXA-48 (45.6%), blaVIM-1 (23.3%), blaNDM-1 (7.8%), blaKPC-3 (6.7%), and blaNDM-5 (6.7%). Forty (44.4%) were resistant to 6 or more antibiotic groups and the most active antibiotics were colistin (98.9%), plazomicin (92.2%) and cefiderocol (92.2%). Four of the seven cefiderocol-resistant isolates belonged to ST167 and six harbored blaNDM. Five of the plazomicin-resistant isolates harbored rmt. IncL plasmids were the most frequent (45.7%) and eight of these harbored blaVIM-1. blaOXA-48 was found in IncF plasmids in eight isolates. Metallo-β-lactamases were more frequent in isolates with resistance to six or more antibiotic groups, with their genes often present on the same plasmid/integron. ST131 isolates were associated with sat and pap virulence genes. This study highlights the genetic versatility of CP-Eco and its potential to disseminate ARGs and cause community and nosocomial infections.