학술논문
FixNCut: single-cell genomics through reversible tissue fixation and dissociation
Document Type
article
Author
Laura Jiménez-Gracia; Domenica Marchese; Juan C. Nieto; Ginevra Caratù; Elisa Melón-Ardanaz; Victoria Gudiño; Sara Roth; Kellie Wise; Natalie K. Ryan; Kirk B. Jensen; Xavier Hernando-Momblona; Joana P. Bernardes; Florian Tran; Laura Katharina Sievers; Stefan Schreiber; Maarten van den Berge; Tessa Kole; Petra L. van der Velde; Martijn C. Nawijn; Philip Rosenstiel; Eduard Batlle; Lisa M. Butler; Ian A. Parish; Jasmine Plummer; Ivo Gut; Azucena Salas; Holger Heyn; Luciano G. Martelotto
Source
Genome Biology, Vol 25, Iss 1, Pp 1-36 (2024)
Subject
Language
English
ISSN
1474-760X
Abstract
Abstract The use of single-cell technologies for clinical applications requires disconnecting sampling from downstream processing steps. Early sample preservation can further increase robustness and reproducibility by avoiding artifacts introduced during specimen handling. We present FixNCut, a methodology for the reversible fixation of tissue followed by dissociation that overcomes current limitations. We applied FixNCut to human and mouse tissues to demonstrate the preservation of RNA integrity, sequencing library complexity, and cellular composition, while diminishing stress-related artifacts. Besides single-cell RNA sequencing, FixNCut is compatible with multiple single-cell and spatial technologies, making it a versatile tool for robust and flexible study designs.