학술논문
Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
Document Type
article
Author
Tomáš Kouba; Tomáš Koval’; Petra Sudzinová; Jiří Pospíšil; Barbora Brezovská; Jarmila Hnilicová; Hana Šanderová; Martina Janoušková; Michaela Šiková; Petr Halada; Michal Sýkora; Ivan Barvík; Jiří Nováček; Mária Trundová; Jarmila Dušková; Tereza Skálová; URee Chon; Katsuhiko S. Murakami; Jan Dohnálek; Libor Krásný
Source
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Subject
Language
English
ISSN
2041-1723
Abstract
The bacterial helicase-like transcription factor HelD associates with the RNA polymerase (RNAP) and recycles stalled transcription complexes. Here, the authors present the cryo-EM structures of three Mycobacterium smegmatis HelD bound RNAP complexes and further show that HelD can prevent the binding of the RNAP core to non-specific DNA and also actively removes RNAP from stalled elongation complexes.