학술논문
Extraction-Free RT-PCR Surveillance Testing and Reporting for SARS-CoV-2
Document Type
article
Author
Patrick R. Carney; Tyler Duellman; Jia-Yi Chan; Lauren Wells; Michael Tessmer; Leah Frater-Rubsam; Molly Zeller; Mark Field; James Speers; Kelly Tyrrell; Luke Thompson; Michael Bondurant; Tami Morin; Tamra Dagnon; Brian Goff; Corissa Runde; Sandra Splinter-Bondurant; Charles Konsitzke; Patrick Kelly; Christopher A. Bradfield; Joshua Hyman
Source
COVID, Vol 3, Iss 7, Pp 1031-1041 (2023)
Subject
Language
English
ISSN
2673-8112
Abstract
The COVID-19 pandemic necessitated sensitive, fast, and inexpensive testing for the virus in 2020 prior to the widespread availability of vaccines. Early testing efforts were limited by bottlenecks on reagents, low-throughput testing options, and the slow return of test results. In this paper, we detail the testing pipeline we established at the University of Wisconsin-Madison for rapid, inexpensive, and sensitive surveillance testing for SARS-CoV-2, and we highlight the strengths of the platform that would allow it to be applied to other disease surveillance projects, SARS-CoV-2 variant testing, or future pandemics. This pipeline can be quickly established for further accreditation and clinical application.