학술논문
Arabidopsis non-specific phospholipase C1: Characterisation and its involvement in response to heat stress
Document Type
article
Author
Source
Frontiers in Plant Science, Vol 6 (2015)
Subject
Language
English
ISSN
1664-462X
Abstract
The Arabidopsis non-specific phospholipase C (NPC) protein family is encoded by the genes NPC1 – NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet.We found that Arabidopsis NPC1 expressed in E. coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type; npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress. Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to heat stress compared with wild-type. These findings suggest that NPC1 is involved in the plant response to heat