학술논문
Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant
Document Type
article
Author
Petra Mlcochova; Dami Collier; Allyson Ritchie; Sonny M. Assennato; Myra Hosmillo; Neha Goel; Bo Meng; Krishna Chatterjee; Vivien Mendoza; Nigel Temperton; Leo Kiss; Leo C. James; Katarzyna A. Ciazynska; Xiaoli Xiong; John A.G. Briggs; James A. Nathan; Federica Mescia; Laura Bergamaschi; Hongyi Zhang; Petros Barmpounakis; Nikos Demeris; Richard Skells; Paul A. Lyons; John Bradley; Steven Baker; Jean Pierre Allain; Kenneth G.C. Smith; Rachel Bousfield; Michael Wilson; Dominic Sparkes; Glenn Amoroso; Effrosyni Gkrania-Klotsas; Susie Hardwick; Adrian Boyle; Ian Goodfellow; Ravindra K. Gupta; Stephen Baker; Gordon Dougan; Ravi Gupta; Paul J. Lehner; Paul Lyons; Nicholas J. Matheson; Mark Toshner; Michael P. Weekes; Nick Brown; Martin Curran; Surendra Palmar; David Enoch; Daniel Chapman; Ashley Shaw; Sherly Jose; Areti Bermperi; Julie Ann Zerrudo; Evgenia Kourampa; Laura Watson; Jieniean Worsley; Caroline Saunders; Ranalie de Jesus; Jason Domingo; Ciro Pasquale; Bensi Vergese; Phoebe Vargas; Marivic Fabiculana; Marlyn Perales; Lee Mynott; Elizabeth Blake; Amy Bates; Anne-Laure Vallier; Alexandra Williams; David Phillips; Edmund Chiu; Alex Overhill; Nicola Ramenatte; Jamal Sipple; Steven Frost; Helena Knock; Richard Hardy; Emily Foster; Fiona Davidson; Viona Rundell; Purity Bundi; Richmond Abeseabe; Sarah Clark; Isabel Vicente; Anne Elmer; Carla Ribeiro; Jenny Kourampa; Jane Kennet; Jane Rowlands; Anne Meadows; Criona O’Brien; Rebecca Rastall; Cherry Crucusio; Sarah Hewitt; Jane Price; Jo Calder; Laura Canna; Ashlea Bucke; Hugo Tordesillas; Julie Harris; Valentina Ruffolo; Barbara Graves; Helen Butcher; Daniela Caputo; Emma Le Gresley; Benjamin J. Dunmore; Jennifer Martin; Ekaterina Legchenko; Carmen Treacy; Christopher Huang; Jennifer Wood; Rachel Sutcliffe; Josh Hodgson; Joy Shih; Stefan Graf; Zhen Tong; Tobias Tilly; Ciara O’Donnell; Kelvin Hunter; Linda Pointon; Nicole Pond; Marta Wylot; Emma Jones; Stuart Fawke; Ben Bullman; Lori Turner; Isobel Jarvis; Ommar Omarjee; Aloka De Sa; Joe Marsden; Ariana Betancourt; Marianne Perera; Maddie Epping; Nathan Richoz; Georgie Bower; Rahul Sharma; Francesca Nice; Oisin Huhn; Natalia Savoinykh Yarkoni; Nika Romashova; Daniel Lewis; Andrew Hinch; Chiara Cossetti; Mateusz Strezlecki; Richard Grenfell; Hannah Stark; Neil Walker; Kathy Stirrups; Nigel Ovington; Eleanor Dewhust; Emily Li; Sofia Papadia; Nathalie Kingston; Andrew Lever; Estee Torok; William Hamilton; Grant Hall; Aminu Jahun; Yasmin Chaudhry; Malte Pinckert; Iliana Georgana; Anna Yakovleva; Laura Caller; Sarah Caddy; Theresa Feltwell; Fahad Khokhar; Luke Meredith; Charlotte Holdcroft; Surendra Parmar
Source
Cell Reports Medicine, Vol 1, Iss 6, Pp 100099- (2020)
Subject
Language
English
ISSN
2666-3791
Abstract
Summary: Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%–50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wild-type or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8–92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity.