학술논문
Single intratesticular injection of blood‐serum‐derived exosomes can potentially alleviate testopathy following testicular torsion
Document Type
article
Author
Source
Animal Models and Experimental Medicine, Vol 5, Iss 4, Pp 362-368 (2022)
Subject
Language
English
ISSN
2576-2095
Abstract
Abstract Background Testicular torsion (TT) is an acute inflammatory process leading to male infertility. Today, anti‐inflammatory effects of exosomes derived from blood serum are used in various laboratory procedures. In the present study, the anti‐inflammatory effects of blood‐serum‐derived exosomes in treatment of acute inflammation following TT in mice were evaluated. Materials and Methods Eighteen male mice were grouped as healthy control, TT, and TT + exosome. TT was induced surgically, and exosomes were extracted from blood serum and administrated by a single intratesticular injection (10 IU). Malondialdehyde (MDA) and Griess assays were used to evaluate the level of oxidative stress. Sperm indices, testosterone (Tes), and apoptotic gene expression (p‐53, Bcl2, and Caspase‐3) were also assessed. H&E and immunohistochemistry (IHC) stainings were used for histopathological investigations. Data analysis was applied by SPSS (v.19) software. Results Oxidative stress and apoptotic genes expression were increased significantly (p