학술논문
Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+T cells of healthy individuals of Indian origin
Document Type
article
Source
Stem Cell Research, Vol 39, Iss , Pp - (2019)
Subject
Language
English
ISSN
1873-5061
Abstract
Human induced pluripotent stem cell-lines (iPSCs) of Indian origin NCCSi005A and NCCSi006A were established by reprogramming of CD4+T cells, isolated from the peripheral blood mononuclear cells (PBMCs) of two healthy female donors. Reprogramming was achieved using integration free, Sendai viral vector system expressing cocktail of transcription factors KOS, hc-MYC and hKLF4. Both the established cell-lines showed alkaline phosphatase activity, expressed stemness markers, exhibited normal female karyotype and displayed potential for tri-lineage differentiation. These two CD4+T cells derived cell-lines represent valuable resource as control iPSC cell lines of Indian origin.