부산대학교 도서관

Chenopodium rubrum L. , is a suitable model plant for studying ontogenesis in vitro as an early flowering species. Culture of intact plants in vitro and antioxidative enzymes detection were performed. Growth pattern to the end of ontogenesis, flowering and seed development are all determined by the photoperiod seedlings experience during induction and evocation of flowering. Different phases of vegetative and reproductive development are characterized by changes in antioxidative enzymes activities. We showed sequential expression of antioxidative enzymes during seed germination. Prior to radicule protrusion, CAT and SOD showed maximal activity, while POD activity appeared later. The highest catalase (CAT) activity was measured at the time of flowering while peroxidases (PODs) are involved in determination of growth and development in accordance with the environmental clues. The absence of some superoxide dismutase (SOD) isoforms could be the indicator of senescence. Seed ageing affect changes in antioxidative status of seeds, germination, seedling growth and flowering.