학술논문
Key mutations in the C-terminus of the HBV surface glycoprotein correlate with lower HBsAg levels in vivo, hinder HBsAg secretion in vitro and reduce HBsAg structural stability in the setting of HBeAg-negative chronic HBV genotype-D infection
Document Type
article
Author
Romina Salpini; Arianna Battisti; Lorenzo Piermatteo; Luca Carioti; Olympia E. Anastasiou; Upkar S. Gill; Domenico Di Carlo; Luna Colagrossi; Leonardo Duca; Ada Bertoli; Katia Yu La Rosa; Lavinia Fabeni; Alessandra Iuvara; Vincenzo Malagnino; Carlotta Cerva; Miriam Lichtner; Claudio M. Mastroianni; Giuseppe Maria De Sanctis; Maurizio Paoloni; Massimo Marignani; Caterina Pasquazzi; Nerio Iapadre; Giustino Parruti; Jacopo Vecchiet; Loredana Sarmati; Massimo Andreoni; Mario Angelico; Sandro Grelli; Patrick T. Kennedy; Jens Verheyen; Stefano Aquaro; Francesca Ceccherini-Silberstein; Carlo Federico Perno; Valentina Svicher
Source
Emerging Microbes and Infections, Vol 9, Iss 1, Pp 928-939 (2020)
Subject
Language
English
ISSN
22221751
2222-1751
2222-1751
Abstract
ABSTRACTIncreasing evidences suggest that HBsAg-production varies across HBV-genotypes. HBsAg C-terminus plays a crucial role for HBsAg-secretion. Here, we evaluate HBsAg-levels in different HBV-genotypes in HBeAg-negative chronic infection, the correlation of specific mutations in HBsAg C-terminus with HBsAg-levels in-vivo, their impact on HBsAg-secretion in-vitro and on structural stability in-silico.HBsAg-levels were investigated in 323 drug-naïve HBeAg-negative patients chronically infected with HBV genotype-D(N = 228), -A(N = 65) and -E(N = 30). Genotype-D was characterized by HBsAg-levels lower than genotype-A and -E (3.3[2.7–3.8]IU/ml; 3.8[3.5–4.2]IU/ml and 3.9[3.7–4.2]IU/ml, P