학술논문
Leishmanicidal and healing effects of 3β,6β,16β-trihydroxy lup-20 (29)-ene isolated from Combretum leprosum on Leishmania braziliensis infection in vitro and in vivo.
Document Type
article
Author
Aline Sombra Santos; Naya Lúcia de Castro Rodrigues; Francisco Rafael Marciano Fonseca; Nathalia Braga Fayão Oliveira; Bianca Oliveira Loucard; Fabíola Fernandes Heredia; Teresa Neuma Albuquerque Gomes Nogueira; Ticiana Monteiro Abreu; Hélcio Silva Dos Santos; Edson Holanda Teixeira; Luzia Kalyne Almeida Moreira Leal; Regis Bernardo Brandim Gomes; Clarissa Romero Teixeira; Maria Jania Teixeira
Source
PLoS ONE, Vol 18, Iss 11, p e0287665 (2023)
Subject
Language
English
ISSN
1932-6203
Abstract
Treatment of cutaneous leishmaniasis depends on drugs that potentially cause serious side effects and resistance. Thus, topical therapies are attractive alternatives to the drugs currently used. 3β, 6β, 16β-trihydroxylup-20 (29)-ene is a lupane triterpene isolated from Combretum leprosum Mart. leaves (CLF-1), with reports of in vitro antileishmanial effect against L. amazonensis and to promote lesion healing in animal model. Herein, we evaluated the in vitro and in vivo antileishmanial and healing effects of CLF-1 against L. braziliensis. CLF-1 treatment showed low toxicity in macrophages and significantly reduced parasite load in vitro. CLF-1 induced higher IL-12 and TNF-α production and more discrete IL-4 and IL-10 production. For in vivo evaluation, a CLF-1 cream formulation was prepared to treat hamsters infected with L. braziliensis. CLF-1 treatment was able to reduce parasite load of the infected skin and lymph node more efficiently than the conventional treatment. Histopathological analysis indicated a strong inflammatory response accompanied by an important healing response. Data from this study indicate that topical CLF-1 treatment was effective and non-toxic in L. braziliensis infected hamsters suggesting its potential for further development as a future therapeutic intervention.