학술논문
Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery
Document Type
article
Author
Anders Lundin; Michelle J. Porritt; Himjyot Jaiswal; Frank Seeliger; Camilla Johansson; Abdel Wahad Bidar; Lukas Badertscher; Sandra Wimberger; Emma J. Davies; Elizabeth Hardaker; Carla P. Martins; Emily James; Therese Admyre; Amir Taheri-Ghahfarokhi; Jenna Bradley; Anna Schantz; Babak Alaeimahabadi; Maryam Clausen; Xiufeng Xu; Lorenz M. Mayr; Roberto Nitsch; Mohammad Bohlooly-Y; Simon T. Barry; Marcello Maresca
Source
Nature Communications, Vol 11, Iss 1, Pp 1-16 (2020)
Subject
Language
English
ISSN
2041-1723
41959140
41959140
Abstract
CRISPR/Cas9 technology has revolutionised the ability of scientists to make genetically modified cells and animal models. Here, the authors make a Tet-On genetically modified mouse using the Streptococcus pyogenes Cas9 and demonstrate that it can be used for generation of mice with lung cancer.