학술논문
TRIM28 and Interacting KRAB-ZNFs Control Self-Renewal of Human Pluripotent Stem Cells through Epigenetic Repression of Pro-differentiation Genes
Document Type
article
Author
Urszula Oleksiewicz; Marta GÅadych; Ayush T. Raman; Holger Heyn; Elisabetta Mereu; Paula Chlebanowska; Anastazja Andrzejewska; Barbara SozaÅska; Neha Samant; Katarzyna FÄ
k; Paulina AuguÅcik; Marcin KosiÅski; Joanna P. Wróblewska; Katarzyna Tomczak; Katarzyna Kulcenty; RafaÅ PÅoski; PrzemysÅaw Biecek; Manel Esteller; Parantu K. Shah; Kunal Rai; Maciej Wiznerowicz
Source
Stem Cell Reports, Vol 9, Iss 6, Pp 2065-2080 (2017)
Subject
Language
English
ISSN
2213-6711
Abstract
Summary: Reprogramming to induced pluripotent stem cells (iPSCs) and differentiation of pluripotent stem cells (PSCs) are regulated by epigenetic machinery. Tripartite motif protein 28 (TRIM28), a universal mediator of Krüppel-associated box domain zinc fingers (KRAB-ZNFs), is known to regulate both processes; however, the exact mechanism and identity of participating KRAB-ZNF genes remain unknown. Here, using a reporter system, we show that TRIM28/KRAB-ZNFs alter DNA methylation patterns in addition to H3K9me3 to cause stable gene repression during reprogramming. Using several expression datasets, we identified KRAB-ZNFs (ZNF114, ZNF483, ZNF589) in the human genome that maintain pluripotency. Moreover, we identified target genes repressed by these KRAB-ZNFs. Mechanistically, we demonstrated that these KRAB-ZNFs directly alter gene expression of important developmental genes by modulating H3K9me3 and DNA methylation of their promoters. In summary, TRIM28 employs KRAB-ZNFs to evoke epigenetic silencing of its target differentiation genes via H3K9me3 and DNA methylation. : Oleksiewicz et al. report that, during somatic cells reprogramming to iPSCs, KRAB-ZNF target genes become stably silenced through H3K9 and DNA methylation. Endogenous KRAB-ZNFs overexpressed in iPSCs, together with TRIM28, were found to participate in the maintenance of pluripotency by targeting and repressing differentiation genes in PSCs. Keywords: reprogramming, induced pluripotent stem cells, epigenetics, TRIM28, KRAB-ZNF repressors, differentiation