학술논문
Genetic analysis of the resistance to rice blast in the BC2F1 population derived from MR263 × Pongsu Seribu 1
Document Type
article
Author
Source
Biotechnology & Biotechnological Equipment, Vol 32, Iss 5, Pp 1134-1140 (2018)
Subject
Language
English
ISSN
1310-2818
1314-3530
13102818
1314-3530
13102818
Abstract
Rice blast disease, caused by Magnaporthe oryzae, is the most important and most devastating rice disease globally. For genetic analysis of resistance to rice blast, the present study used a BC2F1 population developed through marker-assisted backcrossing from a cross between blast susceptible MR263 and blast resistant Pongsu Seribu 1 (PS1). We selected out of 450 markers, 65 polymorphic simple sequence repeat markers, including the Pi gene-based markers, and identified 16 markers associated with blast resistance that showed heterozygous bands in the BC2F1 population. Of the 16 polymorphic markers, only eight (RM5961, RM263, RM163, RM224, RM262, RM168, RM229 and RM169) showed a good fit to the expected segregation genotypic ratio (1:1) for the single dominance gene model (df = 1.0, P