학술논문
Expression of NEDD4L and ENaC in Urinary Extracellular Vesicles in Pre-eclampsia
Document Type
article
Author
Source
Hypertension in Pregnancy, Vol 42, Iss 1 (2023)
Subject
Language
English
ISSN
1064-1955
1525-6065
10641955
1525-6065
10641955
Abstract
Objective To assess changes in expression of renal epithelial sodium channel (ENaC) and NEDD4L, a ubiquitin ligase, in urinary extracellular vesicles (UEV) of pre-eclamptic women compared to normal pregnant controls. Methods Urine was collected from pre-eclamptic women (PE, n = 20) or during normal pregnancy (NP, n = 20). UEV were separated by differential ultracentrifugation. NEDD4L, α-ENaC and γ-ENaC were identified by immunoblotting. Results There was no difference in the expression of NEDD4L (p = 0.17) and α-ENaC (p = 0.10). PE subjects showed increased expression of γ-ENaC by 6.9-fold compared to NP (p