학술논문
Survival gain in glioblastoma patients treated with dendritic cell immunotherapy is associated with increased NK but not CD8+ T cell activation in the presence of adjuvant temozolomide
Document Type
article
Author
Serena Pellegatta; Marica Eoli; Valeria Cuccarini; Elena Anghileri; Bianca Pollo; Sara Pessina; Simona Frigerio; Maura Servida; Lucia Cuppini; Carlo Antozzi; Stefania Cuzzubbo; Cristina Corbetta; Rosina Paterra; Francesco Acerbi; Paolo Ferroli; Francesco DiMeco; Laura Fariselli; Eugenio A. Parati; Maria Grazia Bruzzone; Gaetano Finocchiaro
Source
OncoImmunology, Vol 7, Iss 4 (2018)
Subject
Language
English
ISSN
2162-402X
2162402X
2162402X
Abstract
In a two-stage phase II study, 24 patients with first diagnosis of glioblastoma (GBM) were treated with dendritic cell (DC) immunotherapy associated to standard radiochemotherapy with temozolomide (TMZ) followed by adjuvant TMZ. Three intradermal injections of mature DC loaded with autologous GBM lysate were administered before adjuvant TMZ, while 4 injections were performed during adjuvant TMZ. According to a two-stage Simon design, to proceed to the second stage progression-free survival (PFS) 12 months after surgery was expected in at least 8 cases enrolled in the first stage. Evidence of immune response and interaction with chemotherapy were investigated. After a median follow up of 17.4 months, 9 patients reached PFS12. In these patients (responders, 37.5%), DC vaccination induced a significant, persistent activation of NK cells, whose increased response was significantly associated with prolonged survival. CD8+ T cells underwent rapid expansion and priming but, after the first administration of adjuvant TMZ, failed to generate a memory status. Resistance to TMZ was associated with robust expression of the multidrug resistance protein ABCC3 in NK but not CD8+ T cells. The negative effect of TMZ on the formation of T cell-associated antitumor memory deserves consideration in future clinical trials including immunotherapy.