부산대학교 도서관

Kang Yang,1,* Chengye Liu,1,* Jun Shao,2 Lingxiang Guo,2 Qing Wang,3 Zhaoxiang Meng,1,* Xing Jin,1,* Xianghe Chen4,* 1Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital Affiliated to Yangzhou University, Yangzhou city, Jiangsu Province, People’s Republic of China; 2Cardiovascular Disease Center, Northern Jiangsu People’s Hospital Affiliated to Yangzhou University, Yangzhou city, Jiangsu Province, People’s Republic of China; 3Respiratory Department, Northern Jiangsu People’s Hospital Affiliated to Yangzhou University, Yangzhou city, Jiangsu Province, People’s Republic of China; 4College of Physical Education, Yangzhou University, Yangzhou city, Jiangsu Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xianghe Chen, College of Physical Education, Yangzhou University, Huayang West Road 196, Yangzhou city, Jiangsu Province, People’s Republic of China, Email huashixh@163.com Xing Jin, Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital Affiliated to Yangzhou University, Nantong West Road 98, Yangzhou city, Jiangsu Province, People’s Republic of China, Email yzjinxing@qq.comBackground: Swimming and intermittent fasting can both improve obesity-induced NAFLD, but which of the two is more effective and whether the combination of the two has a superimposed effect is inconclusive.Methods: The model of NAFLD in obese rats was established by a high-fat diet and performed swimming, intermittent fasting, and a combination of both interventions for 8 weeks. Serum lipids and enzyme activity were measured by an automatic biochemical analyzer. Liver morphostructural analysis was observed by transmission electron microscopy, and morphology was observed by HE staining. RT‒PCR was used to detect the mRNA level.Results: Morphology and microstructure of the liver of model rats were impaired, with the upregulation of miR-122-5p, SREBP-1c, FASN and ACC1. Eight weeks of swimming exercise, intermittent fasting and the combination of both attenuate these effects, manifested by the downregulation of miR-122-5p and upregulation of CPT1A mRNA levels. There was no significant stacking effect of the combination of the swimming and intermittent fasting interventions.Conclusion: NAFLD leads to pathology in model rats. Eight weeks of swimming exercise, intermittent fasting and the combination of both can inhibit miR-122-5p and improve hepatic lipid metabolism, while no significant additive effects of combining the interventions were found.Keywords: obesity, NAFLD, swimming, intermittent fasting, miR-122-5p