부산대학교 도서관

Abstract To investigate the effect of dietary l‐Alanyl‐l‐glutamine (Ala‐Gln) supplementation on Amur sturgeon, Acipenser schrenckii, 180 healthy fish (initial body weight 21.23 ± 0.17 g) were divided into two groups (30 fish per tank, each treatment in triplicate) and treated with 0.0% (control) and 1.0% Ala‐Gln for 56 days. The growth performance improved significantly with dietary Ala‐Gln supplementation. Transcriptome profiles of dietary Ala‐Gln supplementation in sturgeon were generated using second‐generation Illumina sequencing technology. A total of 2891 differentially expressed genes (DEGs) were obtained, including 901, which were upregulated, and 1990, which were downregulated, in the treatment group relative to the control group. These genes are mainly involved in the general function, regulation of replication, recombination and repair, signal transduction mechanisms, amino acids and lipid transport and metabolism, and other biological processes. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of DEGs showed that cytokine–cytokine receptor interaction, biosynthesis of unsaturated fatty acids, and beta‐alanine metabolism were the top three most significantly enriched pathways out of the 20 highly enriched pathways detected in A. schrenckii. The expression patterns of DEGs were validated by quantitative real‐time polymerase chain reaction. Our findings provide a comprehensive insight into A. schrenckii genes and pathways with altered expression following dietary Ala‐Gln supplementation.