학술논문
Evaluation of T cell responses to naturally processed variant SARS-CoV-2 spike antigens in individuals following infection or vaccination
Document Type
article
Author
Zixi Yin; Ji-Li Chen; Yongxu Lu; Beibei Wang; Leila Godfrey; Alexander J. Mentzer; Xuan Yao; Guihai Liu; Dannielle Wellington; Yiqi Zhao; Peter A.C. Wing; Wanwisa Dejnirattisa; Piyada Supasa; Chang Liu; Philip Hublitz; Ryan Beveridge; Craig Waugh; Sally-Ann Clark; Kevin Clark; Paul Sopp; Timothy Rostron; Juthathip Mongkolsapaya; Gavin R. Screaton; Graham Ogg; Katie Ewer; Andrew J. Pollard; Sarah Gilbert; Julian C. Knight; Teresa Lambe; Geoffrey L. Smith; Tao Dong; Yanchun Peng
Source
Cell Reports, Vol 42, Iss 5, Pp 112470- (2023)
Subject
Language
English
ISSN
2211-1247
Abstract
Summary: Most existing studies characterizing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cell responses are peptide based. This does not allow evaluation of whether tested peptides are processed and presented canonically. In this study, we use recombinant vaccinia virus (rVACV)-mediated expression of SARS-CoV-2 spike protein and SARS-CoV-2 infection of angiotensin-converting enzyme (ACE)-2-transduced B cell lines to evaluate overall T cell responses in a small cohort of recovered COVID-19 patients and uninfected donors vaccinated with ChAdOx1 nCoV-19. We show that rVACV expression of SARS-CoV-2 antigen can be used as an alternative to SARS-CoV-2 infection to evaluate T cell responses to naturally processed spike antigens. In addition, the rVACV system can be used to evaluate the cross-reactivity of memory T cells to variants of concern (VOCs) and to identify epitope escape mutants. Finally, our data show that both natural infection and vaccination could induce multi-functional T cell responses with overall T cell responses remaining despite the identification of escape mutations.