학술논문
Drug resistance phenotypes and genotypes in Mexico in representative gram-negative species: Results from the infivar network.
Document Type
article
Author
Elvira Garza-González; Paola Bocanegra-Ibarias; Miriam Bobadilla-Del-Valle; Luis Alfredo Ponce-de-León-Garduño; Verónica Esteban-Kenel; Jesus Silva-Sánchez; Ulises Garza-Ramos; Humberto Barrios-Camacho; Luis Esaú López-Jácome; Claudia A Colin-Castro; Rafael Franco-Cendejas; Samantha Flores-Treviño; Rayo Morfín-Otero; Fabian Rojas-Larios; Juan Pablo Mena-Ramírez; María Guadalupe Fong-Camargo; Cecilia Teresita Morales-De-la-Peña; Lourdes García-Mendoza; Elena Victoria Choy-Chang; Laura Karina Aviles-Benitez; José Manuel Feliciano-Guzmán; Eduardo López-Gutiérrez; Mariana Gil-Veloz; Juan Manuel Barajas-Magallón; Efren Aguirre-Burciaga; Laura Isabel López-Moreno; Rebeca Thelma Martínez-Villarreal; Jorge Luis Canizales-Oviedo; Carlos Miguel Cetina-Umaña; Daniel Romero-Romero; Fidencio David Bello-Pazos; Nicolás Rogelio Eric Barlandas-Rendón; Joyarib Yanelli Maldonado-Anicacio; Enrique Bolado-Martínez; Mario Galindo-Méndez; Talia Perez-Vicelis; Norma Alavez-Ramírez; Braulio J Méndez-Sotelo; Juan Francisco Cabriales-Zavala; Yirla Citlali Nava-Pacheco; Martha Irene Moreno-Méndez; Ricardo García-Romo; Aldo Rafael Silva-Gamiño; Ana María Avalos-Aguilera; María Asunción Santiago-Calderón; Maribel López-García; María Del Consuelo Velázquez-Acosta; Dulce Isabel Cobos-Canul; María Del Rosario Vázquez-Larios; Ana Elizabeth Ortiz-Porcayo; Arely Elizabeth Guerrero-Núñez; Jazmín Valero-Guzmán; Alina Aracely Rosales-García; Heidy Leticia Ostos-Cantú; Adrián Camacho-Ortiz
Source
PLoS ONE, Vol 16, Iss 3, p e0248614 (2021)
Subject
Language
English
ISSN
1932-6203
Abstract
AimThis report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum β-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico.Material and methodsA total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the WHONET 5.6 platform. The following clinical isolates recovered from selected specimens were included: carbapenem-resistant Enterobacteriaceae, ESBL or carbapenem-resistant E. coli, and K. pneumoniae, carbapenem-resistant A. baumannii complex, and P. aeruginosa. Strains were genotyped to detect ESBL and/or carbapenemase-encoding genes.ResultsAmong blood isolates, A. baumannii complex showed more than 68% resistance for all antibiotics tested, and among Enterobacteria, E. cloacae complex showed higher resistance to carbapenems. A. baumannii complex showed a higher resistance pattern for respiratory specimens, with only amikacin having a resistance lower than 70%. Among K. pneumoniae isolates, blaTEM, blaSHV, and blaCTX were detected in 68.79%, 72.3%, and 91.9% of isolates, respectively. Among E. coli isolates, blaTEM, blaSHV, and blaCTX were detected in 20.8%, 4.53%, and 85.7% isolates, respectively. For both species, the most frequent genotype was blaCTX-M-15. Among Enterobacteriaceae, the most frequently detected carbapenemase-encoding gene was blaNDM-1 (81.5%), followed by blaOXA-232 (14.8%) and blaoxa-181(7.4%), in A. baumannii was blaOXA-24 (76%) and in P. aeruginosa, was blaIMP (25.3%), followed by blaGES and blaVIM (13.1% each).ConclusionOur study reports that NDM-1 is the most frequent carbapenemase-encoding gene in Mexico in Enterobacteriaceae with the circulation of the oxacillinase genes 181 and 232. KPC, in contrast to other countries in Latin America and the USA, is a rare occurrence. Additionally, a high circulation of ESBL blaCTX-M-15 exists in both E. coli and K. pneumoniae.