학술논문
Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study
Document Type
article
Author
S. Pascual; B. Noble; N. Ahmad-Saeed; C. Aldridge; S. Ambretti; S. Amit; R. Annett; S. A. O'Shea; A. M. Barbui; G. Barlow; L. Barrett; M. Berth; A. Bondi; N. Boran; S. E. Boyd; C. Chaves; M. Clauss; P. Davies; I. T. Dianzo-Delgado; J. Esteban; S. Fuchs; L. Friis-Hansen; D. Goldenberger; A. Kraševac Glaser; J. O. Groonroos; I. Hoffmann; T. Hoffmann; H. Hughes; M. Ivanova; P. Jezek; G. Jones; Z. Ceren Karahan; C. Lass-Flörl; F. Laurent; L. Leach; M. L. Horsbøll Pedersen; C. Loiez; M. Lynch; R. J. Maloney; M. Marsh; O. Milburn; S. Mitchell; L. S. P. Moore; L. Moffat; M. Murdjeva; M. E. Murphy; D. Nayar; G. Nigrisoli; F. O'Sullivan; B. Öz; T. Peach; C. Petridou; M. Prinz; M. Rak; N. Reidy; G. M. Rossolini; A.-L. Roux; P. Ruiz-Garbajosa; K. Saeed; L. Salar-Vidal; C. Salas Venero; M. Selvaratnam; E. Senneville; P. Starzengruber; B. Talbot; V. Taylor; R. Trebše; D. Wearmouth; B. Willinger; M. Wouthuyzen-Bakker; B. Couturier; F. Allantaz
Source
Journal of Bone and Joint Infection, Vol 9, Pp 87-97 (2024)
Subject
Language
English
ISSN
2206-3552
Abstract
Introduction: The BIOFIRE Joint Infection (JI) Panel is a diagnostic tool that uses multiplex-PCR testing to detect microorganisms in synovial fluid specimens from patients suspected of having septic arthritis (SA) on native joints or prosthetic joint infections (PJIs). Methods: A study was conducted across 34 clinical sites in 19 European and Middle Eastern countries from March 2021 to June 2022 to assess the effectiveness of the BIOFIRE JI Panel. Results: A total of 1527 samples were collected from patients suspected of SA or PJI, with an overall agreement of 88.4 % and 85 % respectively between the JI Panel and synovial fluid cultures (SFCs). The JI Panel detected more positive samples and microorganisms than SFC, with a notable difference on Staphylococcus aureus, Streptococcus species, Enterococcus faecalis, Kingella kingae, Neisseria gonorrhoeae, and anaerobic bacteria. The study found that the BIOFIRE JI Panel has a high utility in the real-world clinical setting for suspected SA and PJI, providing diagnostic results in approximately 1 h. The user experience was positive, implying a potential benefit of rapidity of results' turnover in optimising patient management strategies. Conclusion: The study suggests that the BIOFIRE JI Panel could potentially optimise patient management and antimicrobial therapy, thus highlighting its importance in the clinical setting.