학술논문
Genetic profiles of Barrett’s esophagus and esophageal adenocarcinoma in Japanese patients
Document Type
article
Author
Mamoru Tokunaga; Kenichiro Okimoto; Naoki Akizue; Kentaro Ishikawa; Yosuke Hirotsu; Kenji Amemiya; Masayuki Ota; Keisuke Matsusaka; Motoi Nishimura; Kazuyuki Matsushita; Tsubasa Ishikawa; Ariki Nagashima; Wataru Shiratori; Tatsuya Kaneko; Hirotaka Oura; Kengo Kanayama; Yuki Ohta; Takashi Taida; Keiko Saito; Tomoaki Matsumura; Tetsuhiro Chiba; Hitoshi Mochizuki; Makoto Arai; Jun Kato; Jun-ichiro Ikeda; Masao Omata; Naoya Kato
Source
Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Subject
Language
English
ISSN
2045-2322
Abstract
Abstract The genetic characteristics of Barrett’s esophagus (BE) and esophageal adenocarcinoma (EAC) in the Japanese population is unclear. This study aims to investigate the genetic characteristics from nondysplastic BE (NDBE) to early EAC in Japan. Clinical information was collected. Moreover, the genetic profile of NDBE without concurrent dysplasia, early EAC, and surrounding BE were also investigated using endoscopic biopsy samples and formalin-fixed, paraffin-embedded specimens from Japanese patients by targeted next-generation sequencing. Immunohistochemical staining for p53 was also performed for EAC lesions. Targeted NGS was performed for 33 cases with 77 specimens. No significant difference exists in the NDBE group between the number of putative drivers per lesion in the short-segment Barrett’s esophagus (SSBE) and long-segment Barrett’s esophagus (LSBE) [0 (range, 0–1) vs. 0 (range, 0–1). p = 1.00]. TP53 putative drivers were found in two patients (16.7%) with nondysplastic SSBE. TP53 was the majority of putative drivers in both BE adjacent to EAC and EAC, accounting for 66.7% and 66.7%, respectively. More putative drivers per lesion were found in the EAC than in the NDBE group [1 (range, 0–3) vs. 0 (range, 0–1). p