학술논문
T-cell and antibody responses to first BNT162b2 vaccine dose in previously infected and SARS-CoV-2-naive UK health-care workers: a multicentre prospective cohort study
Document Type
article
Author
Adrienn Angyal, PhD; Stephanie Longet, PhD; Shona C Moore, PhD; Rebecca P Payne, DPhil; Adam Harding, MSc; Tom Tipton, PhD; Patpong Rongkard, MSc; Mohammad Ali, MD; Luisa M Hering, MSc; Naomi Meardon, MBChB; James Austin, PhD; Rebecca Brown, PhD; Donal Skelly, PhD; Natalie Gillson, BSc; Sue L Dobson, MSc; Andrew Cross, PhD; Gurjinder Sandhar, MSc; Jonathan A Kilby, MSc; Jessica K Tyerman, BSc; Alexander R Nicols, MSc; Jarmila S Spegarova, PhD; Hema Mehta, DPhil; Hailey Hornsby, MSc; Rachel Whitham, MSc; Christopher P Conlon, ProfPhD; Katie Jeffery, PhD; Philip Goulder, ProfDPhil; John Frater, ProfPhD; Christina Dold, PhD; Matthew Pace, PhD; Ane Ogbe, PhD; Helen Brown, BSc; M Azim Ansari, DPhil; Emily Adland, PhD; Anthony Brown, BSc; Meera Chand, FRCPath; Adrian Shields, PhD; Philippa C Matthews, PhD; Susan Hopkins, PhD; Victoria Hall, PhD; William James, ProfDPhil; Sarah L Rowland-Jones, ProfDM; Paul Klenerman, ProfPhD; Susanna Dunachie, ProfPhD; Alex Richter, ProfPhD; Christopher J A Duncan, DPhil; Eleanor Barnes, ProfPhD; Miles Carroll, ProfPhD; Lance Turtle, PhD; Thushan I de Silva, PhD; Adam Harding; Adam Watson; Adrian Shields; Adrienn Angyal; Ahmed Alhussni; Alex Richter; Alexander Nicols; Alexandra Deeks; Alice Webb-Bridges; Andrew Cross; Ane Ogbe; Anni Jämsén; Anthony Brown; Anu Chawla; Christina Dold; Christopher Duncan; Christopher Conlon; Donal Skelly; Denise O'Donnell; Eleanor Barnes; Emily Adland; Esme Weeks; Gurjinder Sandhar; Hailey Hornsby; Helen Brown; Hema Mehta; Hibatullah Abuelgasim; Huiyuan Xiao; James Austin; Jarmila Spegarova; Jennifer Holmes; Jenny Haworth; Jessica Tyerman; John Frater; Jonathan Kilby; Joseph Cutteridge; Katie Jeffery; Katy Lillie; Lance Turtle; Leigh Romaniuk; Lucy Denly; Luisa Hering; M. Azim Ansari; Matthew Pace; Meera Chand; Miles Carroll; Mohammad Ali; Mwila Kasanyinga; Naomi Meardon; Natalie Gillson; Patpong Rongkard; Paul Klenerman; Philip Goulder; Philippa Matthews; Rachel Whitham; Rebecca Brown; Rebecca Payne; Robert Wilson; Sarah Rowland-Jones; Sarah Thomas; Shona Moore; Siobhan Gardiner; Stephanie Longet; Stephanie Tucker; Sue Dobson; Susan Hopkins; Susanna Dunachie; Syed Adlou; Thushan de Silva; Tom Tipton; Victoria Hall; William James; Allan Lawrie; Nikki Smith; Helena Turton; Amira Zawia; Martin Bayley; Alex Fairman; Kate Harrington; Rosemary Kirk; Louise Marsh; Lisa Watson; Steven Wood; Benjamin Diffey; Chris Jones; Lauren Lett; Gareth Platt; Krishanthi Subramaniam; Daniel Wootton; Brendan Payne; Sophie Hambleton; Sinead Kelly; Judith Marston; Sonia Poolan; Dianne Turner; Muzlifah Haniffa; Emily Stephenson; Sandra Adele; Hossain Delowar Akhter; Senthil Chinnakannan; Catherine de Lara; Timothy Donnison; Carl-Philipp Hackstein; Lian Lee; Nicholas Lim; Tom Malone; Eloise Phillips; Narayan Ramamurthy; Nichola Robinson; Oliver Sampson; David Eyre; Beatrice Simmons; Lizzie Stafford; Alexander Mentzer; Ali Amini; Carolina Arancibia-Cárcamo; Nicholas Provine; Simon Travis; Stavros Dimitriadis; Sile Johnson; Sarah Foulkes; Jameel Khawam; Edgar Wellington; Javier Gilbert-Jaramillo; Michael Knight; Maeva Dupont; Emily Horner; James Thaventhiran; Jeremy Chalk
Source
The Lancet Microbe, Vol 3, Iss 1, Pp e21-e31 (2022)
Subject
Language
English
ISSN
2666-5247
11417935
11417935
Abstract
Summary: Background: Previous infection with SARS-CoV-2 affects the immune response to the first dose of the SARS-CoV-2 vaccine. We aimed to compare SARS-CoV-2-specific T-cell and antibody responses in health-care workers with and without previous SARS-CoV-2 infection following a single dose of the BNT162b2 (tozinameran; Pfizer–BioNTech) mRNA vaccine. Methods: We sampled health-care workers enrolled in the PITCH study across four hospital sites in the UK (Oxford, Liverpool, Newcastle, and Sheffield). All health-care workers aged 18 years or older consenting to participate in this prospective cohort study were included, with no exclusion criteria applied. Blood samples were collected where possible before vaccination and 28 (±7) days following one or two doses (given 3–4 weeks apart) of the BNT162b2 vaccine. Previous infection was determined by a documented SARS-CoV-2-positive RT-PCR result or the presence of positive anti-SARS-CoV-2 nucleocapsid antibodies. We measured spike-specific IgG antibodies and quantified T-cell responses by interferon-γ enzyme-linked immunospot assay in all participants where samples were available at the time of analysis, comparing SARS-CoV-2-naive individuals to those with previous infection. Findings: Between Dec 9, 2020, and Feb 9, 2021, 119 SARS-CoV-2-naive and 145 previously infected health-care workers received one dose, and 25 SARS-CoV-2-naive health-care workers received two doses, of the BNT162b2 vaccine. In previously infected health-care workers, the median time from previous infection to vaccination was 268 days (IQR 232–285). At 28 days (IQR 27–33) after a single dose, the spike-specific T-cell response measured in fresh peripheral blood mononuclear cells (PBMCs) was higher in previously infected (n=76) than in infection-naive (n=45) health-care workers (median 284 [IQR 150–461] vs 55 [IQR 24–132] spot-forming units [SFUs] per 106 PBMCs; p