학술논문
Epigenome-wide scan identifies differentially methylated regions for lung cancer using pre-diagnostic peripheral blood
Document Type
article
Author
Source
Epigenetics, Vol 17, Iss 4, Pp 460-472 (2022)
Subject
Language
English
ISSN
1559-2294
1559-2308
15592294
1559-2308
15592294
Abstract
Background: DNA methylation markers have been associated with lung cancer risk and may identify aetiologically relevant genomic regions, or alternatively, be markers of disease risk factors or biological processes associated with disease development. Methods: In a nested case–control study, we measured blood leukocyte DNA methylation levels in pre-diagnostic samples collected from 430 participants (208 cases; 222 controls) in the 1989 CLUE II cohort. We compared DNA methylation levels with case/control status to identify novel genomic regions, both single CpG sites and differentially methylated regions (DMRs), while controlling for known DNA methylation changes associated with smoking using a previously described pack-years-based smoking methylation score. Stratification analyses were conducted over time from blood draw to diagnosis, histology, and smoking status. Results: We identified 16 single CpG sites and 40 DMRs significantly associated with lung cancer risk (q