부산대학교 도서관

The Kolli hills, a part of Eastern Ghats of India is a treasure of medicinal plants. An attempt is made to gather information about the traditional use of herbs from the local healers. Acanthephippium bicolor Lindley is being used to treat urinary tract infection. Different parts (leaf, bulb and root) are collected in two seasons (summer and winter) and phytochemicals present in them are analyzed. Leaf is found to possess most of the phytochemicals. Hence leaf is selected as study material. Antimicrobial activity of the various solvent extracts (methanol, ethanol, chloroform, acetone, ethyl acetate, benzene and hexane) is screened for both summer and winter samples. Of the 35 organisms studied, Staphylococcus aureus, Streptococcus foecalis, Bacillus cereus, Proteus vulgaris, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Shigella dysenteriae, Escherichia coli, Microsporum audouinii, Microsporum fulvum, Candida albicans and Trichophyton rubrum are found to be sensitive to leaf extracts. The Gram-positive bacteria are found to be more sensitive than Gram-negative bacteria and fungi. The inhibition is found to be more in methanol extract. This proves that the bioactive compounds reside in methanolic extract. When the summer and winter samples are compared for their antimicrobial efficacy, there is no significant difference. This proves that there is no impact of season on antimicrobial activity of this plant. This research proves that Acanthephippium bicolor Lindley would be the best herbal medicine for Urinary Tract Infection and leaves can be used as herbal and scientific medicine throughout the year as there is no seasonal impact.