학술논문
Systematic analysis of human antibody response to ebolavirus glycoprotein shows high prevalence of neutralizing public clonotypes
Document Type
article
Author
Elaine C. Chen; Pavlo Gilchuk; Seth J. Zost; Philipp A. Ilinykh; Elad Binshtein; Kai Huang; Luke Myers; Stefano Bonissone; Samuel Day; Chandrahaas R. Kona; Andrew Trivette; Joseph X. Reidy; Rachel E. Sutton; Christopher Gainza; Summer Diaz; Jazmean K. Williams; Christopher N. Selverian; Edgar Davidson; Erica Ollmann Saphire; Benjamin J. Doranz; Natalie Castellana; Alexander Bukreyev; Robert H. Carnahan; James E. Crowe, Jr.
Source
Cell Reports, Vol 42, Iss 4, Pp 112370- (2023)
Subject
Language
English
ISSN
2211-1247
Abstract
Summary: Understanding the human antibody response to emerging viral pathogens is key to epidemic preparedness. As the size of the B cell response to a pathogenic-virus-protective antigen is poorly defined, we perform deep paired heavy- and light-chain sequencing in Ebola virus glycoprotein (EBOV-GP)-specific memory B cells, allowing analysis of the ebolavirus-specific antibody repertoire both genetically and functionally. This approach facilitates investigation of the molecular and genetic basis for the evolution of cross-reactive antibodies by elucidating germline-encoded properties of antibodies to EBOV and identification of the overlap between antibodies in the memory B cell and serum repertoire. We identify 73 public clonotypes of EBOV, 20% of which encode antibodies with neutralization activity and capacity to protect mice in vivo. This comprehensive analysis of the public and private antibody repertoire provides insight into the molecular basis of the humoral immune response to EBOV GP, which informs the design of vaccines and improved therapeutics.