학술논문
Evaluation of the BD Phoenix Carbapenemase-Producing Organism Panels for the Detection of Carbapenemase Producers in Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa
Document Type
article
Author
Yoselin Paola Correa-León; José Miguel Pérez-Hernández; Bernardo Alfonso Martinez-Guerra; Eduardo Rodríguez-Noriega; Juan Pablo Mena-Ramírez; Eduardo López-Gutiérrez; Luis Esaú López-Jácome; Víctor Antonio Monroy-Colin; Christian Daniel Mireles-Davalos; Cecilia Padilla-Ibarra; María Angelina Quevedo-Ramos; José Manuel Feliciano-Guzmán; Talía Pérez-Vicelis; María del Consuelo Velázquez-Acosta; Melissa Hernández-Durán; Elvira Garza-González
Source
Diagnostics, Vol 13, Iss 22, p 3417 (2023)
Subject
Language
English
ISSN
2075-4418
Abstract
The classification of carbapenemases can help guide therapy. The present study evaluated the performance of the CPO detection test, included in the BD Phoenix™ NMIC-501 panel for the detection and classification of carbapenemases on the representative molecularly characterized strains collection from Mexico. Carbapenem non-susceptible isolates collected in Mexico were included. The clinical isolates (n = 484) comprised Klebsiella pneumoniae (n = 154), Escherichia coli (n = 150), and P. aeruginosa (n = 180). BD Phoenix CPO NMIC-504 and NMIC-501 panels were used for the identification of species, antimicrobial susceptibility tests, and detection of CPOs. For the detection of carbapenemase-encoding genes, E. coli and K. pneumoniae were evaluated using PCR assays for blaNDM-1, blaKPC, blaVIM, blaIMP, and blaOXA-48-like. For P. aeruginosa, blaVIM, blaIMP, and blaGES were detected using PCR. Regarding E. coli, the CPO panels had a sensitivity of 70% and specificity of 83.33% for the detection of a class B carbapenemase (blaNDM in the molecular test). Regarding K. pneumoniae, the panels had a sensitivity of 75% and specificity of 100% for the detection of a class A carbapenemase (blaKPC in the molecular test). The Phoenix NMIC-501 panels are reliable for detecting class B carbapenemases in E. coli. The carbapenemase classification in K. pneumoniae for class A carbapenemases has a high specificity and PPV; thus, a positive result is of high value.