학술논문
Host parameters and mode of infection influence outcome in SARS-CoV-2-infected hamsters
Document Type
article
Author
Bryan D. Griffin; Bryce M. Warner; Mable Chan; Emelissa Valcourt; Nikesh Tailor; Logan Banadyga; Anders Leung; Shihua He; Amrit S. Boese; Jonathan Audet; Wenguang Cao; Estella Moffat; Lauren Garnett; Kevin Tierney; Kaylie N. Tran; Alixandra Albietz; Kathy Manguiat; Geoff Soule; Alexander Bello; Robert Vendramelli; Jessica Lin; Yvon Deschambault; Wenjun Zhu; Heidi Wood; Samira Mubareka; David Safronetz; James E. Strong; Carissa Embury-Hyatt; Darwyn Kobasa
Source
iScience, Vol 24, Iss 12, Pp 103530- (2021)
Subject
Language
English
ISSN
2589-0042
Abstract
Summary: The golden hamster model of SARS-CoV-2 infection recapitulates key characteristics of COVID-19. In this work we examined the influence of the route of exposure, sex, and age on SARS-CoV-2 pathogenesis in hamsters. We report that delivery of SARS-CoV-2 by a low- versus high-volume intranasal or intragastric route results in comparable viral titers in the lung and viral shedding. However, low-volume intranasal exposure results in milder weight loss, whereas intragastric exposure leads to a diminished capacity to regain body weight. Male hamsters, and particularly older male hamsters, display an impaired capacity to recover from illness and delayed viral clearance. These factors were found to influence the nature of the host inflammatory cytokine response but had a minimal effect on the quality and durability of the humoral immune response and susceptibility to re-infection. These data further elucidate key factors that impact pre-clinical challenge studies carried out in the hamster model of COVID-19.