학술논문
Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates.
Document Type
article
Author
Christopher A Cottrell; Jelle van Schooten; Charles A Bowman; Meng Yuan; David Oyen; Mia Shin; Robert Morpurgo; Patricia van der Woude; Mariëlle van Breemen; Jonathan L Torres; Raj Patel; Justin Gross; Leigh M Sewall; Jeffrey Copps; Gabriel Ozorowski; Bartek Nogal; Devin Sok; Eva G Rakasz; Celia Labranche; Vladimir Vigdorovich; Scott Christley; Diane G Carnathan; D Noah Sather; David Montefiori; Guido Silvestri; Dennis R Burton; John P Moore; Ian A Wilson; Rogier W Sanders; Andrew B Ward; Marit J van Gils
Source
PLoS Pathogens, Vol 16, Iss 8, p e1008753 (2020)
Subject
Language
English
ISSN
1553-7366
1553-7374
1553-7374
Abstract
The induction of broad and potent immunity by vaccines is the key focus of research efforts aimed at protecting against HIV-1 infection. Soluble native-like HIV-1 envelope glycoproteins have shown promise as vaccine candidates as they can induce potent autologous neutralizing responses in rabbits and non-human primates. In this study, monoclonal antibodies were isolated and characterized from rhesus macaques immunized with the BG505 SOSIP.664 trimer to better understand vaccine-induced antibody responses. Our studies reveal a diverse landscape of antibodies recognizing immunodominant strain-specific epitopes and non-neutralizing neo-epitopes. Additionally, we isolated a subset of mAbs against an epitope cluster at the gp120-gp41 interface that recognize the highly conserved fusion peptide and the glycan at position 88 and have characteristics akin to several human-derived broadly neutralizing antibodies.