학술논문
Desloratadine, an FDA-approved cationic amphiphilic drug, inhibits SARS-CoV-2 infection in cell culture and primary human nasal epithelial cells by blocking viral entry
Document Type
article
Author
Margot Morin-Dewaele; Sophie Bartier; François Berry; Rozenn Brillet; Dennis Salomón López-Molina; Công Trung Nguyễn; Pascale Maille; Kevin Sereno; Quentin Nevers; Laurent Softic; Jean-Marie Vaugeois; Bruno Louis; Emilie Bequignon; Patrice Bruscella; André Coste; Jean-Michel Pawlotsky; Stéphane Jamain; Abdelhakim Ahmed-Belkacem
Source
Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022)
Subject
Language
English
ISSN
2045-2322
Abstract
Abstract The 2019 global coronavirus (COVID-19) pandemic has brought the world to a grinding halt, highlighting the urgent need for therapeutic and preventive solutions to slow the spread of emerging viruses. The objective of this study was to assess the anti-SARS-CoV-2 effectiveness of 8 FDA-approved cationic amphiphilic drugs (CADs). SARS-CoV-2-infected Vero cells, Calu-3 cells and primary Human Nasal Epithelial Cells (HNEC) were used to investigate the effects of CADs and revealed their antiviral mode of action. Among the CADs tested, desloratadine, a commonly used antiallergic, well-tolerated with no major side effects, potently reduced the production of SARS-CoV-2 RNA in Vero-E6 cells. Interestingly, desloratadine was also effective against HCoV-229E and HCoV-OC43 showing that it possessed broad-spectrum anti-coronavirus activity. Investigation of its mode of action revealed that it targeted an early step of virus lifecycle and blocked SARS-CoV-2 entry through the endosomal pathway. Finally, the ex vivo kinetic of the antiviral effect of desloratadine was evaluated on primary Human Nasal Epithelial Cells (HNEC), showing a significant delay of viral RNA production with a maximal reduction reached after 72 h of treatment. Thus, this treatment could provide a substantial contribution to prophylaxis and systemic therapy of COVID-19 or other coronaviruses infections and requires further studies.