학술논문
Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform
Document Type
article
Author
Source
Communications Biology, Vol 5, Iss 1, Pp 1-13 (2022)
Subject
Language
English
ISSN
2399-3642
Abstract
Riazanski et al describe a platform to capture extracellular vesicles (EVs) using a nanoporous silicon nitride membrane, investigate the expression of NHE1 protein on the surface of EVs and monitor the transport of Na+ and H+ at the single EV level. The authors report a mechanistic function of the proteins found in EVs and specifically identify NHE1 on a single EV, where it maintains pH neutrality within single vesicles.