학술논문

Reproducible, portable, and efficient ancient genome reconstruction with nf-core/eager
Document Type
article
Source
PeerJ, Vol 9, p e10947 (2021)
Subject
Bioinformatics
Palaeogenomics
Ancient DNA
Pipeline
Nextflow
Reproducibility
Medicine
Biology (General)
QH301-705.5
Language
English
ISSN
2167-8359
96976438
Abstract
The broadening utilisation of ancient DNA to address archaeological, palaeontological, and biological questions is resulting in a rising diversity in the size of laboratories and scale of analyses being performed. In the context of this heterogeneous landscape, we present an advanced, and entirely redesigned and extended version of the EAGER pipeline for the analysis of ancient genomic data. This Nextflow pipeline aims to address three main themes: accessibility and adaptability to different computing configurations, reproducibility to ensure robust analytical standards, and updating the pipeline to the latest routine ancient genomic practices. The new version of EAGER has been developed within the nf-core initiative to ensure high-quality software development and maintenance support; contributing to a long-term life-cycle for the pipeline. nf-core/eager will assist in ensuring that a wider range of ancient DNA analyses can be applied by a diverse range of research groups and fields.