학술논문
Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source
Document Type
article
Author
Filip Leonarski; Jie Nan; Zdenek Matej; Quentin Bertrand; Antonia Furrer; Ishkhan Gorgisyan; Monika Bjelčić; Michal Kepa; Hannah Glover; Viktoria Hinger; Thomas Eriksson; Aleksander Cehovin; Mikel Eguiraun; Piero Gasparotto; Aldo Mozzanica; Tobias Weinert; Ana Gonzalez; Jörg Standfuss; Meitian Wang; Thomas Ursby; Florian Dworkowski
Source
IUCrJ, Vol 10, Iss 6, Pp 729-737 (2023)
Subject
Language
English
ISSN
2052-2525
20522525
20522525
Abstract
Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most third-generation synchrotron-based macromolecular crystallography beamlines do not offer the necessary infrastructure yet. Here, a new setup is demonstrated, based on the JUNGFRAU detector and Jungfraujoch data-acquisition system, that enables collection of kilohertz serial crystallography data at fourth-generation synchrotrons. More importantly, it is shown that this setup is capable of collecting multiple-time-point time-resolved protein dynamics at kilohertz rates, allowing the probing of microsecond to second dynamics at synchrotrons in a fraction of the time needed previously. A high-quality complete X-ray dataset was obtained within 1 min from lysozyme microcrystals, and the dynamics of the light-driven sodium-pump membrane protein KR2 with a time resolution of 1 ms could be demonstrated. To make the setup more accessible for researchers, downstream data handling and analysis will be automated to allow on-the-fly spot finding and indexing, as well as data processing.