학술논문
Detection and molecular analysis of Hop latent virus and Hop latent viroid in hop samples from Poland
Document Type
article
Author
Source
Journal für Kulturpflanzen, Vol 66, Iss 7 (2014)
Subject
Language
German
English
English
ISSN
1867-0911
1867-0938
1867-0938
Abstract
Monitoring the occurrence of virus diseases in plants is important for the implementation of early control measures and prevention of further disease spread. In Poland, in 2004 a health programme for hop was started to eliminate viruses and viroids. In 2012/13, in vitro plants, samples from the IUNG-PIB experimental station and commercial hop gardens in Poland were tested for Hop latent virus (HpLV), and Hop latent and Hop stunt viroids (HpLVd and HpSVd). For virus testing, RT-PCR and ELISA methods were used. In order to detect hop viroids, RT-PCR was employed. The overall incidence of HpLV and hop viroids was lower than reported before the start of the programme. Cloning and sequencing revealed that the HpLV and the HpLVd from Polish sources are very similar to the type sequences and the Czech sources. DOI: 10.5073/JfK.2014.07.04, https://doi.org/10.5073/JfK.2014.07.04