학술논문
Quantitative analysis of m6A RNA modification by LC-MS
Document Type
article
Author
Source
STAR Protocols, Vol 2, Iss 3, Pp 100724- (2021)
Subject
Language
English
ISSN
2666-1667
Abstract
Summary: N6-adenosine methylation (m6A) of messenger RNA (mRNA) plays key regulatory roles in gene expression. Accurate measurement of m6A levels is thus critical to understand its dynamic changes in various biological settings. Here, we provide a protocol to quantitate the levels of adenosine and m6A in cellular mRNAs. Using nuclease and phosphatase, we digest mRNA into nucleosides, which are subsequently quantified using liquid chromatography mass spectrometry.For complete details on the use and execution of this protocol, please refer to Cho et al. (2021).