학술논문
A catalogue of biochemically diverse CRISPR-Cas9 orthologs
Document Type
article
Author
Giedrius Gasiunas; Joshua K. Young; Tautvydas Karvelis; Darius Kazlauskas; Tomas Urbaitis; Monika Jasnauskaite; Mantvyda M. Grusyte; Sushmitha Paulraj; Po-Hao Wang; Zhenglin Hou; Shane K. Dooley; Mark Cigan; Clara Alarcon; N. Doane Chilcoat; Greta Bigelyte; Jennifer L. Curcuru; Megumu Mabuchi; Zhiyi Sun; Ryan T. Fuchs; Ezra Schildkraut; Peter R. Weigele; William E. Jack; G. Brett Robb; Česlovas Venclovas; Virginijus Siksnys
Source
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Subject
Language
English
ISSN
2041-1723
Abstract
A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available toolbox of RNA-programmable CRISPR-associated nucleases.