학술논문
Supplementation of an Anthocyanin-Rich Elderberry (Sambucus nigra L.) Extract in FVB/n Mice: A Healthier Alternative to Synthetic Colorants
Document Type
article
Author
Tiago Azevedo; Tiago Ferreira; João Ferreira; Filipa Teixeira; Diana Ferreira; Rita Silva-Reis; Maria João Neuparth; Maria João Pires; Maria de Lurdes Pinto; Rui M. Gil da Costa; Margarida M. S. M. Bastos; Rui Medeiros; Luís Félix; Carlos Venâncio; Maria Inês Dias; Isabel Gaivão; Lillian Barros; Paula A. Oliveira
Source
Applied Sciences, Vol 12, Iss 23, p 11928 (2022)
Subject
Language
English
ISSN
2076-3417
Abstract
Sambucus nigra L., popularly known as elderberry, is renowned for its amazing therapeutic properties, as well as its uses as a food source, in nutraceuticals, and in traditional medicine. This study’s aim was to investigate the effects of an elderberry extract (EE) on mice for 29 days, as well as the safety of the extract when used as a natural colorant. Twenty-four FVB/n female mice (n = 6) were randomly assigned to one of four groups: control, 12 mg/mL EE (EE12), 24 mg/mL EE (EE24), or 48 mg/mL EE (EE48). The predominant anthocyanins detected were cyanidin-3-O-sambubioside and cyanidin-3-O-glucoside. Food and drink intake were similar between groups, with the exception of EE48, who drank significantly less compared with the Control. Biochemical analysis of the liver showed that the changes observed in histological analysis had no pathological significance. The EE, at doses of 24 and 48 mg/mL, significantly reduced the oxidative DNA damage compared with the non-supplemented group. The S. nigra extract showed a favorable toxicological profile, affording it potential to be used in the food industry.