학술논문
Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment
Document Type
article
Author
Andrew J. Percy; Jessica Tamura-Wells; Juan Pablo Albar; Kerman Aloria; Ardeshir Amirkhani; Gabriel D.T. Araujo; Jesus M. Arizmendi; Francisco J. Blanco; Francesc Canals; Jin-Young Cho; Núria Colomé-Calls; Fernando J. Corrales; Gilberto Domont; Guadalupe Espadas; Patricia Fernandez-Puente; Concha Gil; Paul A. Haynes; Maria Luisa Hernáez; Jin Young Kim; Arthur Kopylov; Miguel Marcilla; Mathew J. McKay; Mehdi Mirzaei; Mark P. Molloy; Leanne B. Ohlund; Young-Ki Paik; Alberto Paradela; Mark Raftery; Eduard Sabidó; Lekha Sleno; Daniel Wilffert; Justina C. Wolters; Jong Shin Yoo; Victor Zgoda; Carol E. Parker; Christoph H. Borchers
Source
EuPA Open Proteomics, Vol 8, Iss C, Pp 6-15 (2015)
Subject
Language
English
ISSN
2212-9685
Abstract
The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.