학술논문
Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection
Document Type
article
Author
Smruti Pushalkar; Bidisha Paul; Qianhao Li; Jian Yang; Rebeca Vasconcelos; Shreya Makwana; Juan Muñoz González; Shivm Shah; Chengzhi Xie; Malvin N. Janal; Erica Queiroz; Maria Bederoff; Joshua Leinwand; Julia Solarewicz; Fangxi Xu; Eman Aboseria; Yuqi Guo; Deanna Aguallo; Claudia Gomez; Angela Kamer; Donna Shelley; Yindalon Aphinyanaphongs; Cheryl Barber; Terry Gordon; Patricia Corby; Xin Li; Deepak Saxena
Source
iScience, Vol 23, Iss 3, Pp - (2020)
Subject
Language
English
ISSN
2589-0042
Abstract
Summary: The trend of e-cigarette use among teens is ever increasing. Here we show the dysbiotic oral microbial ecology in e-cigarette users influencing the local host immune environment compared with non-smoker controls and cigarette smokers. Using 16S rRNA high-throughput sequencing, we evaluated 119 human participants, 40 in each of the three cohorts, and found significantly altered beta-diversity in e-cigarette users (p = 0.006) when compared with never smokers or tobacco cigarette smokers. The abundance of Porphyromonas and Veillonella (p = 0.008) was higher among vapers. Interleukin (IL)-6 and IL-1β were highly elevated in e-cigarette users when compared with non-users. Epithelial cell-exposed e-cigarette aerosols were more susceptible for infection. In vitro infection model of premalignant Leuk-1 and malignant cell lines exposed to e-cigarette aerosol and challenged by Porphyromonas gingivalis and Fusobacterium nucleatum resulted in elevated inflammatory response. Our findings for the first time demonstrate that e-cigarette users are more prone to infection. : In Vitro Toxicology; Microbiome; Oral Microbiology Subject Areas: In Vitro Toxicology, Microbiome, Oral Microbiology