학술논문
Epigenomic response to albuterol treatment in asthma-relevant airway epithelial cells
Document Type
article
Author
Javier Perez-Garcia; Maria Pino-Yanes; Elizabeth G. Plender; Jamie L. Everman; Celeste Eng; Nathan D. Jackson; Camille M. Moore; Kenneth B. Beckman; Vivian Medina; Sunita Sharma; Daniel Efrain Winnica; Fernando Holguin; José Rodríguez-Santana; Jesús Villar; Elad Ziv; Max A. Seibold; Esteban G. Burchard
Source
Clinical Epigenetics, Vol 15, Iss 1, Pp 1-16 (2023)
Subject
Language
English
ISSN
1868-7083
Abstract
Abstract Background Albuterol is the first-line asthma medication used in diverse populations. Although DNA methylation (DNAm) is an epigenetic mechanism involved in asthma and bronchodilator drug response (BDR), no study has assessed whether albuterol could induce changes in the airway epithelial methylome. We aimed to characterize albuterol-induced DNAm changes in airway epithelial cells, and assess potential functional consequences and the influence of genetic variation and asthma-related clinical variables. Results We followed a discovery and validation study design to characterize albuterol-induced DNAm changes in paired airway epithelial cultures stimulated in vitro with albuterol. In the discovery phase, an epigenome-wide association study using paired nasal epithelial cultures from Puerto Rican children (n = 97) identified 22 CpGs genome-wide associated with repeated-use albuterol treatment (p