부산대학교 도서관

As an abiotic stress, adverse germination temperatures cause serious disruptions in physiological and biochemical processes involved in seed germination. Using a factorial experiment, we examined the effects of different seed priming treatments on enzymatic and biochemical performances of rice seed germination under different temperatures. Each of the rice genotypes (Hashemi, Sadry-domsefid, IRON-70-7053-7 and NORIN-22) was primed with hydro-hardening, KCl, CaCl2 and ascorbic acid (AsA) and without a priming agent as a control at low (15 ºC), optimum (25 ºC) and high (35 ºC) germination temperatures. The results showed that the enzymatic and biochemical performances of all the rice genotypes were affected by the seed priming agents, especially under the low germination temperature. At 15 ºC, seed priming with AsA was found to be the best agent for the activities of amylase, α-amylase, catalase (CAT), peroxidase (POX), ascorbate peroxidase (APOX) and superoxide dismutase (SOD) as well as the content of soluble sugars in the NORIN-22 genotype, and for protease activity and soluble protein content in the IRON-70-7053-7 genotype. SOD at the low germination temperature and CAT, POX and protease at the optimum and high germination temperatures were the most important enzymes in occurrence of germination potential in terms of seedling length, vigor index, normal seedling rate and germination rate. Under the priming agents, the highest changes in normal seedling rate were observed at the low and optimum germination temperatures by AsA priming in the Hashemi and NORIN-22 genotypes, and at the high germination temperature under KCl priming in the Hashemi genotype.