학술논문
Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines
Document Type
article
Author
Julie Van Coillie; Tamas Pongracz; Tonći Šuštić; Wenjun Wang; Jan Nouta; Mathieu Le Gars; Sofie Keijzer; Federica Linty; Olvi Cristianawati; Jim B.D. Keijser; Remco Visser; Lonneke A. van Vught; Marleen A. Slim; Niels van Mourik; Merel J. Smit; Adam Sander; David E. Schmidt; Maurice Steenhuis; Theo Rispens; Morten A. Nielsen; Benjamin G. Mordmüller; Alexander P.J. Vlaar; C. Ellen van der Schoot; Ramon Roozendaal; Manfred Wuhrer; Gestur Vidarsson; Brent Appelman; Diederik van de Beek; Marije K. Bomers; Justin de Brabander; Matthijs C. Brouwer; David T.P. Buis; Nora Chekrouni; Marit J. van Gils; Menno D. de Jong; Ayesha H.A. Lavell; Sabine E. Olie; Edgar J.G. Peters; Tom D.Y. Reijnders; Michiel Schinkel; Alex R. Schuurman; Jonne J. Sikkens; Yvo M. Smulders; Joost W. Wiersinga; Antinori Spinello; Cinzia Bassoli; Giovanna Bestetti; Mario Corbellino; Alice Covizzi; Angelica Lupo; Laura Milazzo; Marco Schiuma; Alessandro Torre; Willem A. de Jongh; Ali Salanti; Thor G. Theander; Matthew B.B. McCall; Meral Esen
Source
iScience, Vol 26, Iss 9, Pp 107619- (2023)
Subject
Language
English
ISSN
2589-0042
Abstract
Summary: IgG antibodies are important mediators of vaccine-induced immunity through complement- and Fc receptor-dependent effector functions. Both are influenced by the composition of the conserved N-linked glycan located in the IgG Fc domain. Here, we compared the anti-Spike (S) IgG1 Fc glycosylation profiles in response to mRNA, adenoviral, and protein-based COVID-19 vaccines by mass spectrometry (MS). All vaccines induced a transient increase of antigen-specific IgG1 Fc galactosylation and sialylation. An initial, transient increase of afucosylated IgG was induced by membrane-encoding S protein formulations. A fucose-sensitive ELISA for antigen-specific IgG (FEASI) exploiting FcγRIIIa affinity for afucosylated IgG was used as an orthogonal method to confirm the LC-MS-based afucosylation readout. Our data suggest that vaccine-induced anti-S IgG glycosylation is dynamic, and although variation is seen between different vaccine platforms and individuals, the evolution of glycosylation patterns display marked overlaps.