학술논문
Multi-level targeting of the phosphatidylinositol-3-kinase pathway in non-small cell lung cancer cells.
Document Type
article
Author
Source
PLoS ONE, Vol 7, Iss 2, p e31331 (2012)
Subject
Language
English
ISSN
1932-6203
Abstract
We assessed expression of p85 and p110α PI3K subunits in non-small cell lung cancer (NSCLC) specimens and the association with mTOR expression, and studied effects of targeting the PI3K/AKT/mTOR pathway in NSCLC cell lines.Using Automated Quantitative Analysis we quantified expression of PI3K subunits in two cohorts of 190 and 168 NSCLC specimens and correlated it with mTOR expression. We studied effects of two PI3K inhibitors, LY294002 and NVP-BKM120, alone and in combination with rapamycin in 6 NSCLC cell lines. We assessed activity of a dual PI3K/mTOR inhibitor, NVP-BEZ235 alone and with an EGFR inhibitor.p85 and p110α tend to be co-expressed (p