학술논문
MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography
Document Type
article
Author
Max T. B. Clabbers; Susannah Holmes; Timothy W. Muusse; Parimala R. Vajjhala; Sara J. Thygesen; Alpeshkumar K. Malde; Dominic J. B. Hunter; Tristan I. Croll; Leonie Flueckiger; Jeffrey D. Nanson; Md. Habibur Rahaman; Andrew Aquila; Mark S. Hunter; Mengning Liang; Chun Hong Yoon; Jingjing Zhao; Nadia A. Zatsepin; Brian Abbey; Emma Sierecki; Yann Gambin; Katryn J. Stacey; Connie Darmanin; Bostjan Kobe; Hongyi Xu; Thomas Ve
Source
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Subject
Language
English
ISSN
2041-1723
Abstract
MAL and MyD88 are downstream adaptors of Toll-like receptors (TLR) and the MAL TIR domain forms filaments in vitro, which in turn nucleate the assembly of crystalline arrays of the MyD88 TIR domain. Here, the authors present the structure of these MyD88 TIR crystalline arrays solved by both microcrystal electron diffraction and serial femtosecond crystallography, and they show with mutagenesis experiments that MyD88 interface residues are important for TLR4 signaling in vivo.