학술논문
Characterization of the humoral immune response to the EBV proteome in extranodal NK/T-cell lymphoma
Document Type
article
Author
Zhiwei Liu; Yomani D. Sarathkumara; John K. C. Chan; Yok-Lam Kwong; Tai Hing Lam; Dennis Kai Ming Ip; Brian C.-H. Chiu; Jun Xu; Yu-Chieh Su; Carla Proietti; Martha M. Cooper; Kelly J. Yu; Bryan Bassig; Raymond Liang; Wei Hu; Bu-Tian Ji; Anna E. Coghill; Ruth M. Pfeiffer; Allan Hildesheim; Nathaniel Rothman; Denise L. Doolan; Qing Lan
Source
Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
Subject
Language
English
ISSN
2045-2322
Abstract
Abstract Extranodal natural killer/T-cell lymphoma (NKTCL) is an aggressive malignancy that has been etiologically linked to Epstein-Barr virus (EBV) infection, with EBV gene transcripts identified in almost all cases. However, the humoral immune response to EBV in NKTCL patients has not been well characterized. We examined the antibody response to EBV in plasma samples from 51 NKTCL cases and 154 controls from Hong Kong and Taiwan who were part of the multi-center, hospital-based AsiaLymph case–control study. The EBV-directed serological response was characterized using a protein microarray that measured IgG and IgA antibodies against 202 protein sequences representing the entire EBV proteome. We analyzed 157 IgG antibodies and 127 IgA antibodies that fulfilled quality control requirements. Associations between EBV serology and NKTCL status were disproportionately observed for IgG rather than IgA antibodies. Nine anti-EBV IgG responses were significantly elevated in NKTCL cases compared with controls and had ORshighest vs. lowest tertile > 6.0 (Bonferroni-corrected P-values